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fc fusion vector  (InvivoGen)


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    Structured Review

    InvivoGen fc fusion vector
    Fc Fusion Vector, supplied by InvivoGen, used in various techniques. Bioz Stars score: 96/100, based on 214 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pfuse+higg1+fc2+vector/pFUSE-hIgG1-Fc2/us12612446-2372-2-7
    Average 96 stars, based on 214 article reviews
    fc fusion vector - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Construct:

    Article Title: Genetically surface-modified Escherichia coli outer membrane vesicles targeting MUC1 antigen in cancer cells
    Article Snippet: To produce recombinant SpyTag-scFv SM3 , pSpyTag-scFv SM3 was constructed by the Gibson assembly technique using NEBuilder® HiFi DNA Assembly Master Mix (New England Biolabs, USA). .. The SpyTag [ ], GS-linker, and anti-MUC1 scFv namely SM3 genes with a N-terminal His-tag [ ] were constructed in the 5′ Eco RI and 3′ Nhe I sites on pFUSE-hIgG1-Fc2 vector (Invivogen, USA). ..

    Article Title: Heterogeneity of tethered agonist signaling in adhesion G protein-coupled receptors.
    Article Snippet: Resource Heterogeneity of tethered agonist signaling in adhesion G protein-coupled receptors

    Article Title: Genetically surface-modified Escherichia coli outer membrane vesicles targeting MUC1 antigen in cancer cells.
    Article Snippet: To produce recombinant SpyTag-scFvSM3, pSpyTag-scFvSM3 was constructed by the Gibson assembly technique using NEBuilder® HiFi DNA Assembly Master Mix (New England Biolabs, USA). .. The SpyTag [8], GS-linker, and anti-MUC1 scFv namely SM3 genes with a N-terminal His-tag [27] were constructed in the 5′ EcoRI and 3′ NheI sites on pFUSE-hIgG1-Fc2 vector (Invivogen, USA). ..

    Plasmid Preparation:

    Article Title: A nanobody-enzyme fusion protein targeting PD-L1 and sialic acid exerts anti-tumor effects by C-type lectin pathway-mediated tumor associated macrophages repolarizing.
    Article Snippet: Aberrant sialylated glycosylation in the tumor microenvironment is a novel immune suppression pathway, which has garnered significant attention as a targetable glycoimmune checkpoint for cancer immunotherapy to address the dilemma of existing therapies.. However, rational drug design and in-depth mechanistic studies are urgently required for tumor sialic acid to become valuable glycoimmune targets.. In this study, we explored the positive correlation of PD-L1 and sialyltransferase expression in clinical colorectal cancer tissues and identified their mutual regulation effects in macrophages.

    Article Title: A Luciferase-Based Approach for Functional Screening of 5′ and 3′ Untranslated Regions of the mRNA Component for mRNA Vaccines
    Article Snippet: .. The PCR product was digested with EcoRI/AvrII and cloned in frame with the interleukin-2 signal sequence at the EcoRI/NheI sites of the pFUSE-hIgG1-Fc2 vector (InvivoGen, San Diego, CA, USA). .. The resulting plasmid encoding RBD-Fc was used to transiently transfect FreeStyle 293-F cells in the serum-free FreeStyle 293 expression medium (Thermo Fisher Scientific, USA) using linear PEI 25K reagent (Polysciences, Warrington, PA, USA).

    Article Title: Heterogeneity of tethered agonist signaling in adhesion G protein-coupled receptors.
    Article Snippet: Resource Heterogeneity of tethered agonist signaling in adhesion G protein-coupled receptors

    Article Title: Early fusion intermediate of ACE2-using coronavirus spike acting as an antiviral target.
    Article Snippet: Article Early fusion intermediate of ACE2-using coronavirus spike acting as an antiviral target

    Article Title: A nanobody-enzyme fusion protein targeting PD-L1 and sialic acid exerts anti-tumor effects by affecting tumor associated macrophages
    Article Snippet: Endotoxins were removed using a high-capacity endotoxin removal spin kit (88275, Thermo Fisher). .. Gene encoding human Siglec9 (amino acid residues 18–347) was cloned into the pFUSE-hIgG1-Fc2 vector (InvivoGen) for expression using HEK293F cells. .. Siglec9-Fc protein was purified by Protein A Sepharose (17127901, Cytiva).

    Expressing:

    Article Title: A nanobody-enzyme fusion protein targeting PD-L1 and sialic acid exerts anti-tumor effects by C-type lectin pathway-mediated tumor associated macrophages repolarizing.
    Article Snippet: Aberrant sialylated glycosylation in the tumor microenvironment is a novel immune suppression pathway, which has garnered significant attention as a targetable glycoimmune checkpoint for cancer immunotherapy to address the dilemma of existing therapies.. However, rational drug design and in-depth mechanistic studies are urgently required for tumor sialic acid to become valuable glycoimmune targets.. In this study, we explored the positive correlation of PD-L1 and sialyltransferase expression in clinical colorectal cancer tissues and identified their mutual regulation effects in macrophages.

    Article Title: A nanobody-enzyme fusion protein targeting PD-L1 and sialic acid exerts anti-tumor effects by affecting tumor associated macrophages
    Article Snippet: Endotoxins were removed using a high-capacity endotoxin removal spin kit (88275, Thermo Fisher). .. Gene encoding human Siglec9 (amino acid residues 18–347) was cloned into the pFUSE-hIgG1-Fc2 vector (InvivoGen) for expression using HEK293F cells. .. Siglec9-Fc protein was purified by Protein A Sepharose (17127901, Cytiva).

    Polymerase Chain Reaction:

    Article Title: A Luciferase-Based Approach for Functional Screening of 5′ and 3′ Untranslated Regions of the mRNA Component for mRNA Vaccines
    Article Snippet: .. The PCR product was digested with EcoRI/AvrII and cloned in frame with the interleukin-2 signal sequence at the EcoRI/NheI sites of the pFUSE-hIgG1-Fc2 vector (InvivoGen, San Diego, CA, USA). .. The resulting plasmid encoding RBD-Fc was used to transiently transfect FreeStyle 293-F cells in the serum-free FreeStyle 293 expression medium (Thermo Fisher Scientific, USA) using linear PEI 25K reagent (Polysciences, Warrington, PA, USA).

    Clone Assay:

    Article Title: A Luciferase-Based Approach for Functional Screening of 5′ and 3′ Untranslated Regions of the mRNA Component for mRNA Vaccines
    Article Snippet: .. The PCR product was digested with EcoRI/AvrII and cloned in frame with the interleukin-2 signal sequence at the EcoRI/NheI sites of the pFUSE-hIgG1-Fc2 vector (InvivoGen, San Diego, CA, USA). .. The resulting plasmid encoding RBD-Fc was used to transiently transfect FreeStyle 293-F cells in the serum-free FreeStyle 293 expression medium (Thermo Fisher Scientific, USA) using linear PEI 25K reagent (Polysciences, Warrington, PA, USA).

    Article Title: Early fusion intermediate of ACE2-using coronavirus spike acting as an antiviral target.
    Article Snippet: Article Early fusion intermediate of ACE2-using coronavirus spike acting as an antiviral target

    Article Title: A nanobody-enzyme fusion protein targeting PD-L1 and sialic acid exerts anti-tumor effects by affecting tumor associated macrophages
    Article Snippet: Endotoxins were removed using a high-capacity endotoxin removal spin kit (88275, Thermo Fisher). .. Gene encoding human Siglec9 (amino acid residues 18–347) was cloned into the pFUSE-hIgG1-Fc2 vector (InvivoGen) for expression using HEK293F cells. .. Siglec9-Fc protein was purified by Protein A Sepharose (17127901, Cytiva).

    Sequencing:

    Article Title: A Luciferase-Based Approach for Functional Screening of 5′ and 3′ Untranslated Regions of the mRNA Component for mRNA Vaccines
    Article Snippet: .. The PCR product was digested with EcoRI/AvrII and cloned in frame with the interleukin-2 signal sequence at the EcoRI/NheI sites of the pFUSE-hIgG1-Fc2 vector (InvivoGen, San Diego, CA, USA). .. The resulting plasmid encoding RBD-Fc was used to transiently transfect FreeStyle 293-F cells in the serum-free FreeStyle 293 expression medium (Thermo Fisher Scientific, USA) using linear PEI 25K reagent (Polysciences, Warrington, PA, USA).



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